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Supreme Antibody from AI-Powered Discovery Platform
XupremAb™ is XtalPi's antibody discovery platform that combines the best of wet-lab techniques and cutting-edge AI technology
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Combine the best of AI and wet labAI is a powerful technology, and its impact is maximized when combined with wet lab. We have built a fully-functional antibody lab with a cross-disciplinary team of AI scientists and experimental biologists. This synergy creates new opportunities that cannot be achieved through wet lab or dry lab techniques alone.
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Tap into greater search space for more quality hitsTo lay a solid foundation for any discovery campaign, it's essential to have as many diverse, high-quality hits as possible at the start. We use AI to enhance existing experimental techniques or let AI take the lead to expand our search space for hits.
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Utilize AI to make antibody engineering more efficient, enabling multi-objective optimizationWe want to reduce the guesswork and random mutagenesis in antibody engineering. AI helps us explore a larger mutant space and account for multiple properties when selecting candidates.
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Instill excellent developability into every moleculeDevelopability is an essential property that we try to capture in the discovery phase. A combination of in vitro and in silico methods allows us to assess developability at ultra high-throughput and reasonable cost.
XupremAb™ is our one-stop solution for antibody discovery that incorporates AI throughout the whole process. The platform can be accessed as a whole, where we can take a partner's project all the way from target to preclinical candidates, or it can be accessed as individual sub-platforms.
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Antibody Generation
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XtraDoma™
Access Extra Leads on Our Upgraded Hybridoma Platform
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Ultra High-throughput, Rapid Screening of Immune Repertoire Powered by NGS & AI
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XpeedPlay™
Ultra High-speed Hit Generation Through Phage Display, Featuring AI-Powered Precision Library
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XuperNovo™
Next-gen Superstar Molecule, Created de novo
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Next-generation Humanized Mouse Platform
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Antibody Engineering
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AI-driven Humanization & Deimmunization
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AI-driven Affinity Modulation
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AI-driven Developability Optimization
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Antibody Developability
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AI-Powered One-Stop Antibody Developability Assessment
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Case Studies
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Tap into greater search space for more quality hits
Case 1: In silico screening of millions of antibody sequences in the immune repertoire
XploreSeq™ routinely sequences millions of BCRs and identifies hits with high confidence (left).
In this project, XploreSeq™ predicted 717 binders, from which 48 were randomly chosen for expression and testing. 66.7% (32/48) were confirmed to be binders, and only 18.6% (6/32) binders overlap with hybridoma hits (right).
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Utilize AI to make antibody engineering more efficient, enabling multi-objective optimization
Case 2: Bi-directional affinity tuning of a lead antibody
Goal: tuning the affinity of a lead antibody both upwards and downwards to provide more options for bispecifics development.
Our AI model generated 3 buckets of designed mutants with predicted affinities of:
1) significant increase (target: 10x),
2) mild increase (target: 3x) and
3) mild decrease (target: -3x) vs. WT
When expressed and tested, these mutants show affinities that correspond to their targeted ranges. (Shown on the left)
1 round of design-make-test of 96 mutants to arrive at a panel of highly optimized leads with KD that span 3 orders of magnitude and optimal developability profiles.
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Instill excellent developability into every molecule
Case 3: XcelDevTM Silico evaluates and ranks 30+ candidates in terms of developability
36 hits that had passed binding and functional screening were analyzed by XcelDevTM Silico. 6 properties of each antibody was predicted and scored on a scale of 0-1 (displayed in color gradient).
An overall developability score was calculated to rank all 36 hits. To verify the effectiveness of the ranking, the top 7 (in the red box) were expressed and subject to a battery of in vitro developability assays. All 7 performed well in these assays, their Tm, SEC, AC-SINS and HIC results shown below.
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XcelDev Silico results for 36 antibodies
Wet lab results for top 7 candidates
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XtalPi is keenly aware that every research partner has unique needs and priorities, and is happy to create a customized solution to optimize your research program
